Mouse cloning perfected? Human cloning not so far away
http://motherboard.vice.com/blog/scientists-perfect-mouse-re-cloning-to-the-25th-generation the cite is:
Sayaka Wakayama, Takashi Kohda, Haruko Obokata, Mikiko Tokoro, Chong Li, Yukari Terashita, Eiji Mizutani, Van Thuan Nguyen, Satoshi Kishigami, Fumitoshi Ishino and Teruhiko Wakayama "Successful serial cloning in the mouse over multiple generations." Cell Stem Cell, 2013, doi:org/10.1016/j.stem.2013.01.005
Abstract
Previous studies of serial cloning in animals showed a decrease in efficiency over repeated iterations and a failure in all species after a few generations. This limitation led to the suggestion that repeated recloning might be inherently impossible because of the accumulation of lethal genetic or epigenetic abnormalities. However, we have now succeeded in carrying out repeated recloning in themouse through a somatic cell nuclear transfer method that includes a histone deacetylase inhibitor. The cloning efficiency didnotdecreaseover 25 generations, and, todate,we have obtained more than 500 viable offspring from a single original donor mouse. The reprogramming efficiency also did not increase over repeated rounds of nuclear transfer, and we did not see the accumula- tion of reprogramming errors or clone-specific abnor- malities. Therefore, our results show that repeated iterative recloning is possible and suggest that, with adequately efficient techniques, it may be possible to reclone animals indefinitely.